Not gonna work! For anything bootable, it must have a master boot record, volume boot record, and contain an operating system or a program that can be executed alongwith a live file creation timestamp is needed.
If that wasn't the case, nobody would had to go through Rufus etc. tools to create usb pen drive instead they would have instead unzipped the iso and boot directly!
@chungus whats the issue buying or at least sourcing an enclosure? Its pretty cheap and always comes handy in such situations.
This way any sata storage can come to the rescue.